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Ever considering that its inception, the internet has replaced the panorama of human studies on how we engage with each other and knowledge via carrier infrastructures through a variety of computing units. This interweaving surroundings is now changing into ever extra embedded into units and platforms that combine seamlessly on how we are living, either in our operating or relaxation time.
Die Geschichte der Menschheit offenbart sich in den Biografien ihrer wichtigsten Vertreter, zumindest bisher. Seit 1927 ernennt das US-amerikanische Nachrichtenmagazin TIME jedes Jahr die "Person des Jahres", die nach Ansicht der Jury die Welt im jeweiligen Jahr maßgeblich verändert oder bewegt hat. Nach beispielsweise Roosevelt, Adenauer, Churchill und Johannes Paul II.
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Lomonosov Academy of Fine Chemical Technology, 86, Vernadsky prospekt, Moscow 119571 Russian Federation 3 Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, 630090, Russian Federation Abstract: Cationic lipid vesicles and polypeptides represent common non-viral gene delivery systems for in vitro and in vivo applications. New non-viral vectors for targeted gene delivery, namely, mono-, di- and tricholesterol derivatives of oligoethyleneimine, glycolipids and chitosan derivatives are reported in this chapter.
Testing of genotoxicity, cytotoxicity and gene transfer activity against transformed monolayer and suspension cell cultures is carried out for all of these mediators of gene transfer. Experimental results show that GLYCOLIPID VI containing a lactose residue, which was used to form liposomes for gene delivery into tissues (using 14 C-adenosine-labeled or plasmid DNA), expressed the affinity of corresponding lipoplexes for kidney, liver, and spleen tissues. GLYCOLIPID VI is a prospective tool for designing new generation of nonviral vectors for targeted gene delivery to tissues.
In vitro cell culture studies and in vivo experiments have proved the MICRO AND NANO SYSTEMS IN BIOMEDICINE AND DRUG DELIVERY 17 potential of nanocarriers in overcoming mucosal barriers such as intestinal nasal and ocular barriers. Recently Dinauer et al (2005) prepared gelatin nanoparticles and antibodies specific for the CD3 antigen of lymphocytic cells were conjugated to the nanoparticle surface. Cellular uptake and effective internalization of antibodyconjugated nanoparticles into CD3 expressing cells were examined.